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Monday, April 20, 2020 | History

3 edition of Cytochemical Analysis of Mammalian Sperm Membranes (Progress in Histochemistry and Cytochemistry) found in the catalog.

Cytochemical Analysis of Mammalian Sperm Membranes (Progress in Histochemistry and Cytochemistry)

F. Sinowatz

Cytochemical Analysis of Mammalian Sperm Membranes (Progress in Histochemistry and Cytochemistry)

  • 295 Want to read
  • 27 Currently reading

Published by Lubrecht & Cramer, Limited .
Written in English

    Subjects:
  • Chemistry - General,
  • Science

  • The Physical Object
    FormatHardcover
    ID Numbers
    Open LibraryOL11309498M
    ISBN 100895742942
    ISBN 109780895742940

    Differentiation and subcellular structure of mammalian sperm 5 The sperm equatorial segment and equatorial subsegment 6 Relocalization of sperm proteins to the EqS 10 Possible mechanisms regulating sperm protein translocation to the EqS 11 Lateral diffusion The process. The acrosomal reaction normally takes place in the ampulla of the fallopian tube (site of fertilization) when the sperm penetrates the secondary oocyte. A few events precede the actual acrosome sperm cell acquires a "hyperactive motility pattern" by which its flagellum produces vigorous whip-like movements that propel the sperm through the cervical canal and uterine. To inhibit fertilisation by more than one sperm (a condition known as polyspermy), eggs have developed preventative mechanisms known as blocks to polyspermy. The block at the level of the egg extracellular coat (the zona pellucida in mammals, the vitelline envelope in non-mammals) has been well characterised in many different animal species and the block at the level of the egg plasma membrane Cited by:   The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. It is widely Cited by: 8.

    Sperm membrane protein relocation is a key process in human and mammalian reproduction. During sperm acrosome reaction (AR), some key molecules responsible for binding and fusion with the egg plasma membrane have to be relocated to the sperm surface and even more specifically to the equatorial segment, where fusion process takes a place.


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Cytochemical Analysis of Mammalian Sperm Membranes (Progress in Histochemistry and Cytochemistry) by F. Sinowatz Download PDF EPUB FB2

Cytochemical Analysis of Mammalian Sperm Membranes Author links open overlay panel Dr. Sinowatz (Professor) 1 Dr. J.K. Voglmayr (Professor) 2 Dr.

H.-J. Gabius (Privatdozent) 3 Dr. A.-E. Friess (Privatdozent) 4Cited by: Cytochemical analysis of mammalian sperm membranes. Stuttgart ; New York: Cytochemical Analysis of Mammalian Sperm Membranes book, (OCoLC) Material Type: Internet resource: Document Type: Book, Internet Resource: All Authors / Contributors: Fred Sinowatz.

cytochemical studies of mammalian tissues* i. isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material by george h. hogeboom, walter c. schneider,t and george e. pallade. Sexual reproduction requires the fusion of sperm cell and oocyte during fertilization to produce the diploid zygote.

In mammals complex changes in the plasma membrane of the sperm cell are involved in this process. Sperm cells have unusual membranes compared to those of somatic by: Mammalian spermatozoa exhibit considerable species differences in their size and shape, yet they all possess the same set of cellular organelles assembled on a common architectural theme.

The Cited by: 2. In this book, I discuss the events that occur in the normal sperm head and govern the structure-function relationship from the time of spermatogenesis to that of fertilization or egg activation. mammals complex changes in the plasma membrane of the sperm cell are involved in this process.

Sperm cells have unusual membranes compared to those of somatic cells. After leaving the testes, sperm cells cease plasma membrane lipid and protein synthesis, and vesicle mediated transport.

Gordon M: Cytochemical analysis of the membranes of the mammalian sperm head. In: Male Reproductive System. Yates RD, Gordon M (ed) New York, Masson, pp 15–33, Cited by: 4.

Parameters for sperm cryosurvival were similar in sperm cooled to either +5 °C or -5 °C, except in the percentage of hyper-fluid membranes which was lower (P. Regulation of membrane stability and the acrosome reaction in mammalian sperm.

Nolan JP(1), Hammerstedt RH. Author information: (1)Life Sciences Division, Los Alamos National Laboratory, New MexicoUSA. Membrane destabilization is an essential step in the process of membrane by: 1. Biol Reprod.

Feb;6(1) Cytochemical demonstration of acrosomal proteinase in mammalian and avian spermatozoa by a silver proteinate by: how are mammalian sperm cells produced and incubated at a lower temperature than body temp. by parasympathetic contraction of muscular tunic in the scrotum to elevate or lower testes from the Cytochemical Analysis of Mammalian Sperm Membranes book.

housing testes in scrotum outside the major body cavity results in the tissue with lower temperature. Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple sperm Allison J.

GardnerA and Janice P. EvansA,B ADepartment of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MDUSA.

BCorresponding File Size: KB. compare the Cytochemical Analysis of Mammalian Sperm Membranes book value of different in situ Cytochemical Analysis of Mammalian Sperm Membranes book tests for sperm chromatin structure.

In addition, the methods were applied to rat and boar spermiogenesis models. The sperm heads with abnor-mal versus normal chromatin structure were specified as orange-red versus green by the AO method, blue versus Cytochemical Analysis of Mammalian Sperm Membranes book by the AB. Cytochemical Analysis of Mammalian Sperm Membranes F.

Sinowatz, J.K. Voglmayr, H.-J. Gabius, A.-E. Friess Progress in Histochemistry and Cytochemistry 19 (4), III Usually a pool of unpolymerized G-actin in between the acrosome and nucleus used to drive the sperm membrane into the egg surface. Neck and midpiece.

behind the sperm head and contains the centriole and the mitochondria final stages of mammalian sperm maturation, doesnt occur until sperm has been inside female reproductive tract for a.

The ultimate goal of a mammalian sperm is the transmission of paternal genome to the next generation. To achieve this goal, mammalian spermatozoa are very specialized cells, which have a precise cellular design in dependence on the evolutionary reproductive strategy chosen by each by: 4.

Diffusion on Mammalian Spermatozoa.- Constraints on Sperm Geometry.- Lipid Diffusion on Mammalian Spermatozoa.- Changes in Sperm Plasma Membrane Lipid Diffusibility during Spermatogenesis, Maturation, and Capacitation.- Causes of Nondiffusing Lipid and the Question of Lipid Domains.- Protein Diffusion on Mammalian.

@article{osti_, title = {Studies on trypsin-like enzymes in sperm and early embryos}, author = {Penn, A}, abstractNote = {Results are reported from a study of acrosomal proteinase, a trypsin-like enzyme (TLE), found in the acrosome of all eutherian mammals studied to date.

It has been implicated in the dissolution of a passage for the sperm through the zona pellucida of the egg, a step. Sperm Biology represents the first analysis of the evolutionary significance of sperm phenotypes and derived sperm traits and the possible selection pressures responsible for sperm-egg coevolution.

An understanding of sperm evolution is fast developing and promises to shed light on many topics from basic reproductive biology to the evolutionary process itself as well as the sperm proteome 5/5(1). COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus.

The mammalian zona pellucida. The zona pellucida (ZP) is a porous extracellular matrix that surrounds the oocyte (Dunbar, et al.,Wassarman and Litscher, ).In the most widely accepted models of gamete interaction, the zona pellucida plays a critical role in tethering spermatozoa, and inducing the release of their acrosomal contents (Bleil and Wassarman, ).Cited by: 2.

The Long, Winding Tale of Sperm Science (Montgomerie notes this in the book Sperm Biology: Sperm cells vary incredibly across the animal kingdom.

This single fruit fly Author: Laura Poppick. Sperm Biology represents the first analysis of the evolutionary significance of sperm phenotypes and derived sperm traits and the possible selection pressures responsible for sperm-egg coevolution.

An understanding of sperm evolution is fast developing and promises to shed light on many topics from basic reproductive biology to the evolutionary process itself as well as the sperm proteome.

its why guys have balls, they hang out of the body and keep the sperm at a lower temperature than the rest of the body is at. the warmer the body, the further away they'll go. when it's cold out though, they'll squeeze up into the body. this way they can keep the sperm at just the perfect temp.

To assess establishment of the membrane block to polyspermy, eggs were examined for their susceptibility to be fertilized when challenged with sperm after activation treatment. Eggs were inseminated in 10 µl insemination drops containing 10 eggs Cited by: Review Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer RAMIRO ALBERIO1*, VALERI ZAKHARTCHENKO 1, JAN MOTLIK2 and ECKHARD WOLF 1Department of Molecular Animal Breeding and Genetics, Ludwig-Maximilian University, Oberschleissheim, Germany and 2Institute of Animal Physiology and Genetics, Czech Academy of Cited by:   The zona pellucida is an extracellular matrix surrounding the mammalian egg where species-specific gamete recognition and signaling occur.

Pig zona pellucida were isolated in large amounts and used as an affinity matrix for detergent-solubilized. Sperm chromatin structure sperm nucleus words: male infertility, human sperm structure, vacuoles, digital.

The morphology of the sperm head has often been correlated with the outcome of in vitro. Although the majority of the DNA in human sperm chromatin is bound by these proteins, a small percentage retains a nucleosomal-like.

damaged membranes, an intact or damaged acrosomal membrane, and high or low mitochondrial potential. The results show that bulls have a low amount of sperm with intact membranes at puberty, and the sperm show low motility, vigor, and wave motion; however, in bulls at early sexual maturity, the integrity of the sperm membrane increased Cited by: 1.

Fertilization is a fundamental process in sexual reproduction, creating a new individual through the combination of male and female gametes 1,2,3,4.

The IZUMO1 sperm membrane protCited by: Date: 12 April Source: I did the diagram myself based on the one found on the book "Gray's anatomy" 36th edition, Williams & Warwick, ; and a diagram found of the review "Formation and organization of the mammalian sperm head" from Kiyotaka Toshimori and Chizuro : Mariana Ruiz Villarreal spermatozoa.

In mammalian cells, it is likely that most protein translation takes place on polysomes, rather than via mRNA translation by a single ribosome. The biochemical analysis of gene expression and regulation benefits from the ability to isolate and characterize these very large polysome by: 8.

Membrane Fluidity in Biology, Volume 4: Cellular Aspects provides a unique interpretation of membrane and cellular activity, as well as cellular aspects of membrane fluidity. This book discusses the influence of membrane fluidity on enzyme activity, function of the sarcoplasmic reticulum in skeletal muscle, role of cholesterol in membrane Book Edition: 1.

Carbohydrates are candidates for the basis of species-selective interaction of gametes during mammalian fertilization.

In this study, we sought to clarify the roles of sugar residues in the species-selective, sperm–oocyte interaction in pigs and cattle. Acrosome-intact porcine and bovine sperm exhibited their strongest binding affinities for β-Gal and α-Man residues, by: 7.

Notes on Sperm Cell Morphology Most of what we know about the effects of abnormal sperm on fertility in animals is derived from studies on bull sperm. However, the effects of sperm cell defects are probably similar regardless of the species involved.

Sperm cell defects can beFile Size: 55KB. By: Christine Burks & Elysse Stone The "Sperm Cell" entering the egg. Middle- with many mitochondria for energy Sperm take 2 ½ months to grow to maturity in the testes meiosis-dna replication during cell division Spermatogonium-A cell produced at an early stage in the formation.

Events after fertilisation have been carefully studied in the last decades. However, there are still several questions to be clarified in relation to the signalling pathway initiated by the sperm, the identification of proteins or factors involved in the activation of the arrested oocyte, and the inactivation of specific molecules involved in the meiotic by: sperm.

Finally, an ORFeome library engineered for use with the Gateway® recombination system expedites cloning into yeast two-hybrid vectors to test for direct interactions (10,11).

This chapter describes sperm and oocyte isolation procedures designed for routine practice in the lab. The procedures are biased toward applications requir. Active sperm-borne oocyte-activating factor(s) (SOAF) appears during transformation of the round spermatid into the spermatozoon.

The action of SOAF is not highly species-specific: mouse oocytes are activated by injection of spermatozoa from foreign species, such as the hamster, rabbit, pig, human, and even by:. Pdf. Condensation of DNA by Folded Bull Protamine—The pdf goal of the present study was to determine whether purified bull protamine (BP) is able to condense DNA in vitro in a manner similar to that observed in native mammalian sperm cell chromatin.

One of the outstanding features of BP is a high percentage of cysteine residues (Fig. 1A).The hypothesis that sperm plasma membrane and oocyte membranes carry complementary molecules involved in a multistep fusion process has been validated by studies of adhesion molecules in sperm-oocyte interaction in a number of animal models ().

The species-specificity of sperm-oocyte recognition and binding primarily resides at the level of. University of North Carolina ebook Charlotte. (, October 11). Advance achieved in dry preservation of mammalian sperm cells. ScienceDaily. Retrieved April 6, from